Assistant Engineer (M/F)

Réf. 924236 - publié le 20 septembre 2024

Informations générales

Missions

Institut Curie vous propose une offre d'emploi dans les domaines Santé - Social, Hôpitaux, Médecine à Paris.

Laboratory:
The CNRS UMR144 "CELL BIOLOGY AND CANCER", investigates cell compartment biogenesis and molecular mechanisms governing both normal cellular functions and pathologies. Within this context, the "DYNAMICS OF INTRA-CELLULAR ORGANIZATION" team studies intra-cellular trafficking in mammalian cells and contributes to the development of biotechnological tools in cell biology ( https://institut-curie.org/team/perez ).
Position Overview:
We are seeking an Assistant Engineer with a background in biochemistry and molecular biology to contribute to ongoing scientific projects. These projects are focused on the biochemical and structural characterization of medically important proteins, including human protein fragments, single domain antibodies, artificially designed proteins, growth factors, and more. The role will involve the production of recombinant proteins and protein complexes using established protocols, as well as the development of new methodologies. Additionally, responsibilities include plasmid, cell and protein inventory management, media and reagent preparation, lab documentation, troubleshooting, and adaptation to the collaborative laboratory environment. There are opportunities for cross-training in complementary techniques if required for specific projects.
Key Responsibilities:
- Amplification, purification, and maintaining of plasmid DNA collection.
- Molecular cloning.
- Expression and purification of recombinant proteins at analytical and preparative scales.
- Preparation of samples for in vitro protein binding assays and structural studies.
- Detailed record-keeping and report generation for all procedures and results.
- Summarization and presentation of data at internal meetings.
- Contribute to preparation and maintaining of laboratory stocks.


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Profil

Candidate profile

Training and Experience:
• At least a bachelor in biological sciences, biochemistry, biotechnology, or a related discipline.
• Some experience in bacteria culture, recombinant protein production and purification (IMAC, affinity chromatography, gel filtration, etc.), protein quantification by spectrophotometry, SDS-PAGE.
• Some experience in molecular cloning.
• Basic calculations: preparation of solutions and buffers, weight and molar concentrations.
• Usage of MS Office applications (Word, PowerPoint, Excel), DNA/protein sequence analysis tools.
Training can be provided for some missing experiences.

Skills and Abilities:
• Communication in English, both verbally and in writing
• Ability to work collaboratively on multiple projects maintaining clear communication of progress and results with team members
• Careful adherence to established experimental protocols.
• Attention to details, commitment to data integrity, and timely completion of tasks, including accurate report generation.

All our opportunities are open to people with disabilities.

Contract information


• Type of contract: Fixed-term contract
• Starting date: As soon as possible
• Duration: until the end of 2025
• Working hours: Full-time
• Remuneration: In accordance with current Institut Curie salary grids, according to qualification
• Location: Paris, 12 rue Lhomond


Contact

Interested candidates are encouraged to submit their CV, a letter of motivation, and references
Application deadline: until a suitable candidate is found
Publication date: 20th September 2024



Institut Curie is an inclusive, equal opportunity employer and is dedicated to the highest standards of research integrity.
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Nom du recruteur : Beetween | Réf : 0itki7zr0r-1